This is consistent with 63% of melanoma metastases exhibiting nuclear TFE3 staining.69 While the high nuclear MITF in melanoma 2 correlated with the lowest total TFEB staining, because the same sections were not probed with both antibodies, the relationship between TFEB and MITF expression and subcellular localization in individual cells in vivo remains uncertain. The gene discussed is TFE3; the disease is melanoma.