Similar to findings in C57BL/6 mice (Fig. 2), VCD-induced AOF treatment in 3xTg-AD mice led to increased plasma FSH levels (Supplementary Fig. 16a) and prolonged oestrous cycles beginning around the 6th–7th cycle posttreatment (Fig. 7a, right panel). The gene discussed is BRD2; the disease is Alzheimer disease.