Next, we performed small interfering RNA (siRNA)-mediated knock-down of NOVA1 in the BC cell line MCF7, followed by bulk RNAseq, and found that ΔPSI values in cancer cells observed between ER+ BC versus TNBC showed a significant positive correlation with those obtained from wild-type versus NOVA1 knockdown conditions (Pearson correlation: r = 0.15, P = 0.028; Fig. 2G). The gene discussed is ESR1; the disease is cancer.