To further investigate whether DAPK1 affects tau phosphorylation and accumulation by modulating tau SUMOylation in vivo, we developed a mouse model of tauopathy by stereotactic injection of rAAV expressing hTau-mCherry into the CA3 region of the hippocampus to mimic the pathological accumulation of tau in the brain. This evidence concerns the gene DAPK1 and tauopathy.