MAPT and Alzheimer disease: Since tau SUMOylation increases tau phosphorylation and accumulation [7], we examined total tau as well as the phosphorylation of tau at AD-related sites including AT8 (Ser202/Thr205), Thr231 (pT231-Tau), Ser262 (pS262-Tau), and Ser396 (pS396-Tau), and found that both total tau and phosphorylated tau at all sites were lower in DAPK1-KO neurons than in WT neurons (Fig. 5D, E).