This study demonstrated that the A allele and AA genotype of the GLIS3 rs7034200C/A variant, along with the AA/TT combined genotype of the GLIS3 rs7034200C/A and ADRB3 rs4994T/C SNPs, were associated with an increased risk of developing GDM among pregnant Chinese women. This evidence concerns the gene GLIS3 and gestational diabetes.