Full-length RPGRIP1 amplicons (3.8 kb) amplified by reverse-transcription PCR (RT-PCR) from LCA-2 organoid cDNA were sequenced by Oxford Nanopore Technology (ONT) and aligned to RPGRIP1 reference sequence NM_020366.4 (Figure 2B). Here, RPGRIP1 is linked to Leber congenital amaurosis.