Additionally, although IL-1β and IL-17 are not directly used as early diagnostic indicators, their mechanism of promoting inflammation by disrupting the intestinal tight junction (TJ) barrier (IL-1β increases luminal antigen penetration, while IL-17 directly damages intestinal cell junctions) is closely associated with the key pathogenesis of NEC (TJ barrier defects). This evidence concerns the gene IL1B and necrotizing enterocolitis.