Bioinformatics analysis also indicated elevated SCD expression in osteosarcoma cells compared to mesenchymal stem cells (MSCs) (Fig. 6C), a finding supported by PCR and western blot results from human bone MSCs (hBMSCs) and human osteosarcoma cell lines, including 143B, U2OS, and HOS (Fig. 6D and E). Here, SCD is linked to osteosarcoma.