NLRP6 and infection: In support of the latter hypothesis, NLRP6 activation was only detectable at 3-4 h post infection in both NLRP6tg HIEC-6 and NLRP6 expressing GFP-ASCtg HEK293T cells (Figs. 1B and 2E), at which time Listeria had already replicated around 10-fold (Fig. EV3H).