Since our findings had shown that secondary infection and the escape from double-membrane vacuoles were crucial for NLRP6 activation, we wondered whether NLRP6 specifically recognizes the lysis of double-membrane vacuoles over the lysis of single-membrane vacuoles, or whether the sheer number of escaping bacteria during late timepoints triggers NLRP6 activation. This evidence concerns the gene NLRP6 and infection.