Western diet (WD)-fed MC4R-KO mice and Gubra-Amylin non-alcoholic steatohepatitis (GAN) diet-fed mice models have been reported to highly resemble human MASH in terms of obesity, dyslipidemia, insulin resistance, liver injury, steatosis, and fibrosis21,26,27. This evidence concerns the gene INS and metabolic dysfunction-associated steatohepatitis.