At last, we also further choose several genes mRNA levels by ISH, the results also showed a significantly upregulation of Axin2, Sox1a, Nkx2.2b, Atoh1a and Isl1. All these findings in our KDM5A-KO zebrafish model were consistent with those in NTD mice model, indicating that KDM5A knockdown disrupts processes involved in neurodevelopment again. This evidence concerns the gene KDM5A and neural tube defect.