The present study provided in vitro evidence showing that SINO prevented NF-κB activation in LPS-induced AMs as revealed by down-regulation of the phosphorylation of NF-κB p65 and its inhibitory factor IκBα, which suggested that NF-κB inactivation was involved in the mechanism of SINO on LPS-induced ALI. The gene discussed is NFKB1; the disease is acute respiratory distress syndrome.