To further verify whether the seven N-glycosidic sugar chains (N-glycans) in the base segment of the human NANOG gene are involved in the molecular quality control of NANOG protein, we cloned and expressed the NANOG protein encoding 305 amino acids, and constructed seven mutant expression vectors corresponding to the deletion of N-glycosylation sites, in which the N-X-T (S) encoding the N-glycosylation site was mutated to A-X-T (S), and then constructed the NANOG-WT cell model in CD133 + cells in HCT116 and LoVo colon cancer cells. Here, NANOG is linked to colonic neoplasm.