NANOG and malignant colon neoplasm: In this study, through the analysis of NANOG amino acid sequence, we preliminarily revealed whether N-glycosylation in the carboxyl terminus of human NANOG protein is involved in the quality control of Nanog protein, and further verified to clarify whether a specific N-glycosidic sugar chain (N-glycans) site is involved in regulating the maintenance of stemness of colon cancer precursor cells.