Our results revealed that caspase-8 was coprecipitated with RIG-I and RIPK1 upon ZIKV infection (Fig. 3G), and that ASC physically binds with RIG-I, caspase-1, and NLRP3 (Fig. 3G), suggesting that ZIKV infection could promote interaction among caspase-8, RIPK1, RIG-I, NLRP3, caspase-1, and ASC to form the PANotosome, which is essential for the activation of PANoptosis. This evidence concerns the gene CASP8 and Zika virus infectious disease.