This is consistent with the study by Senasri et al. [27], which reported that B. gonionotus did not show infection with metacercariae of liver fluke, and displayed low infection density in fish and minimal recovery of metacercariae (0.00%) within 14 days of receiving a diet supplemented with vitamin C. Additionally, throughout the experimental period, the SOD levels were the lowest in the group that did not receive cannabis supplementation, while the fish fed cannabis leaves at a concentration of 2.0% for 14 days demonstrated the highest SOD value of 1497.96 U/g FW. The gene discussed is SOD1; the disease is infection.