Future research on TSC22D3 can focus on: (1) exploring its regulatory network and interactions with osteogenic pathways like WNT, TGF-β, and BMP; (2) creating a conditional knockout mouse model to study its role in bone formation and regeneration; (3) investigating its epigenetic regulation, such as methylation or noncoding RNA; (4) developing molecular interventions targeting TSC22D3 for potential applications in osteoporosis or bone defect treatment. This evidence concerns the gene TGFB1 and osteoporosis.