The mutation frequency top 5 genes in the CNS LS-related tumor group were TP53 (AF 91.18%), SETD2 (AF 67.65%), NF1 (AF 63.24%), SMARCA4 (AF 52.94%) and PDGFRA (AF 51.47%); in the Non-CNS LS-related tumor group were MUC16 (AF 75.21%), ACVR2A (AF 64.96%), FAT4 (AF 64.96%), LRP1B (AF 63.25%) and CIC (AF 61.54%); and in the Non-CNS LS-unrelated tumor group were TP53 (AF 52.83%), EGFR (AF 35.85%), MUC16 (AF 33.96%), CDKN2B (AF 20.75%) and LPR1B (AF 20.75%). The gene discussed is MUC16; the disease is neoplasm.