Given the significant increase in CD8+ TSCM cells observed in the scRNA-seq study (Fig. 2D), we collected peripheral blood samples from a discovery cohort of 39 MDD patients and 39 psychiatrically healthy controls (cohort 3), which was specifically assembled to investigate changes in the proportions of various T cell subsets (Supplementary Fig. 3A and Supplementary Data 9, 10). The gene discussed is CD8A; the disease is major depressive disorder.