Subsequently, the IIF-HEp 2 could be employed in cases of discrepancy with the clinical presentation, with the identification panel and also in cases where the antigen is not represented in the screening assay, particularly some nucleolar antigens associated with systemic sclerosis, antigens linked to inflammatory myopathies and the case of the DFS70 Antigen (19–22). The gene discussed is PSIP1; the disease is systemic sclerosis.