By applying TCRseq together with scRNAseq on CT26 MMRd tumor infiltrating cells, we observed expansion of multiple clones of CD4+ and CD8+ T cells following treatment with the combination of PD-1 blockade and vaccination compared to untreated or anti-PD-1 treated tumors (Figure 4 D, Extended Figure 5). The gene discussed is CD8A; the disease is neoplasm.