Flow cytometry analysis of CD62L, CCR7, and CD45RA, which are stem-like markers, and CD45RO, a T cell memory marker 28,29 revealed that B7H3.BC CAR-T cells had higher expression of CD62L, CCR7, and CD45RA and lower expression of CD45RO compared to MGA271 and Hu8H9 CAR-T cells on day 14 of culture (Figure S2D, E). This evidence concerns the gene CCR7 and breast cancer.