To investigate whether FTD-linked MAPT mutations affect the nucleolar functions of tau, we used a tetracycline-inducible SH-SY5Y cell model developed by the Buée lab that expresses 4-repeat (4R) tau harbouring the P301S or S305N mutations, or an empty vector (EV) control 47 (Fig. 1B). The gene discussed is MAPT; the disease is frontotemporal dementia.