To functionally interrogate Kdm8 in neoplasms initiated by oncogenic Kras and Trp53 inactivation, we built upon a previous approach 58 that enables the induction of spontaneous PDA in the Trp53flox/flox;R26LSL-Tomato;H11LSL-Cas9 mice (PTC; Figure 2A) by delivering an engineered adeno-associated virus (AAV) into the adult murine pancreas through a retrograde pancreatic ductal injection procedure 57. Here, KDM8 is linked to Patent ductus arteriosus.