In contrast, HPV-negative tumors were characterized by increased fibroblasts (in plasma and tumor-immune enriched neighborhoods), macrophages (in endothelial, fibroblast, granulocyte, PPSC, and other enriched neighborhoods), and PD-L1+ CD4 T- cells (in CD4 T- cell–enriched neighborhoods) (Fig. S2D; Suppl. This evidence concerns the gene CD274 and neoplasm.