Genomic compartment switching, topologically associated domain fusion, and novel enhancer–promoter interactions subsequently enhance the expression of PCOS-related genes, including CYP17A1 and CYP11A1. In mice, ectopic expression of Pkm2 in female GCs consistently presented PCOS-like traits, such as interrupted estrous cycles, hyperandrogenism, and so on. Here, CYP11A1 is linked to polycystic ovary syndrome.