PKM and polycystic ovary syndrome: To investigate whether PKM2 upregulation is crucial for PCOS development, we generated a mouse line that specifically ectopically expressed Pkm2 in ovarian GCs by crossing Rosa26-loxP-Stop-loxP-Pkm2-tdTomato (R26-LSL-Pkm2-tdTomato) knock-in mice with AMH-Cre knock-in mice (Fig. 3a).