PGK1 and polycystic ovary syndrome: In a previous study, we demonstrated that the glycolysis gene PGK1 reprogrammed gene expression in GCs and contributed to the onset of PCOS.15 Additionally, elevated expression of growth differentiation factor 8 (GDF8) caused ovulation failure by disrupting normal glucose metabolism in GCs.50 Given the frequent co-occurrence of glucose metabolism disorders in patients with PCOS, our objective was to elucidate how dysregulation of glucose metabolism, especially metabolic enzymes, leads to PCOS.