In a previous study, we demonstrated that the glycolysis gene PGK1 reprogrammed gene expression in GCs and contributed to the onset of PCOS.15 Additionally, elevated expression of growth differentiation factor 8 (GDF8) caused ovulation failure by disrupting normal glucose metabolism in GCs.50 Given the frequent co-occurrence of glucose metabolism disorders in patients with PCOS, our objective was to elucidate how dysregulation of glucose metabolism, especially metabolic enzymes, leads to PCOS. This evidence concerns the gene MSTN and glucose metabolism disease.