We have previously shown that the GCs microenvironment is disrupted by dysregulated expression of PGK1, a key glycolytic gene, which contributes to PCOS development.15 To further dissect the relationship between the dysregulation of glycolysis and PCOS pathogenesis, in this study, Mendelian randomization (MR) analysis was used to investigate the causal associations between genetically predicted differences in glycolysis enzymes and the risk of PCOS and demonstrated that PKM2 might contribute to the genetic determinants of PCOS. The gene discussed is PGK1; the disease is polycystic ovary syndrome.