We confirmed the RNA-seq results via qPCR; in TEPP-46-treated mouse ovary GCs, PCOS-associated genes, such as Pkm2, Wnt4, Klhl30, Avpi1, Padi3, Cyp11a1 and Cyp17a1, were downregulated to WT mouse levels (Fig. 6j). This evidence concerns the gene WNT4 and polycystic ovary syndrome.