2 × 106 MACS-sorted CD3+ T cells from spleens of naive 12-week-old prediabetic NOD mice, conditioned prediabetic NOD mice (12 weeks after conditioning), or prediabetic NOD:B6 chimeras (45% donor T cell chimerism; 12 weeks after HCT) were transferred into normoglycemic naive 7- to 8-week-old NOD Rag1-KO female mice and monitored for diabetes incidence. Here, RAG1 is linked to diabetes mellitus.