In summary, this characterisation reveals that the TDP43ΔNLS mice used in this study displayed TDP‐43 pathology, motor deficits and morphological changes in spinal cord MNs which are consistent with human ALS pathology (Trist et al. 2022) and previous findings using this mouse model (Bak et al. 2023). This evidence concerns the gene TARDBP and amyotrophic lateral sclerosis.