CD4+ naïve T cells were isolated from C57BL/6 mouse spleens, activated with anti‐CD3/CD28 magnetic beads in the presence of interleukin (IL)‐2, and then co‐cultured with the murine HCC cell line Hepa53.4 to recapitulate the TME (Figure S4E, Supporting Information). The gene discussed is IL2; the disease is hepatocellular carcinoma.