To determine whether the effect of puerarin on Ti‐Tregs is conserved in human cells, we isolated high‐purity nTregs from healthy donors’ peripheral blood using magnetic bead sorting for CD4+CD25+CD127− cells, cultured them with IL‐2, and then co‐cultured them with HepG2, a HCC cell line, to mimic TME‐induced Ti‐Tregs (Figure S8A, Supporting Information). This evidence concerns the gene IL7R and hepatocellular carcinoma.