PANX1 and neuroblastoma: We first tested the impact of pre-treatment with amiloride (1 h; 300 μM) or vehicle (DMSO) on ATP-induced internalization of PANX1-EGFP constitutively expressed in murine neuroblastoma N2a cells using an analysis paradigm established in our previous studies (Boyce and Swayne, 2017; Boyce et al., 2015).