Biochemical indicators showed significantly higher levels of White blood cell (WBC), Hematocrit (HCT), Alanine aminotransferase (ALT), Aspartate aminotransferase (AST), Lactate, LDH, Triglyceride (TG), Blood urea nitrogen (BUN), Creatinine, D-dimer, Median Systemic inflammatory response syndrome (SIRS), Median APACHE II, Median hospital days, Median ICU days, and Mortality in the PRF group. This evidence concerns the gene GPT and systemic inflammatory response syndrome.