CALCRL and granular corneal dystrophy type II: Rheobase measurements revealed significant hyperexcitability of spinal Calcrl+ neurons in ACD model (Sham: 41.83 ± 3.81 pA vs. ACD: 28.75 ± 3.54 pA; p = 0.0464, n = 38 neurons from 5 mice for sham, group, n = 40 neurons from 5 mice for ACD group; Fig 3A and 3B), despite unaltered resting membrane potential (Sham: −57.14 ± 1.13 mV vs. ACD: 55.64 ± 1.07 pA; p = 0.3579, n = 30 neurons from 5 mice for sham group, n = 49 neurons from 5 mice for ACD group; Fig 3C).