To investigate the potential mechanisms by which ISGs may interfere with viral infection at later points in the life cycle, we analyzed published RNA-seq data from SARS-CoV-2-infected Calu-3 and A549-ACE2 cell lines, both of which are human lung adenocarcinoma cells and the latter of which overexpresses ACE2, the cellular receptor for SARS-CoV-2 (28). This evidence concerns the gene ACE2 and viral infectious disease.