To confirm these findings in the context of authentic virus infection, we examined the effect of GBP5 or GBP5-C583A overexpression on S protein processing and infection with SARS-CoV-2 in HEK 293T cells expressing the SARS-CoV-2 co-receptors ACE2 and TMPRSS2. This evidence concerns the gene TMPRSS2 and viral infectious disease.