On day 7 after cerebral ischemia reperfusion, the number of VEGF-positive cells in the I/R + TFDM group was significantly higher than that in the I/R group (p < 0.01), with positive cells mainly concentrated in the regions adjacent to microvessels, small arteries, and small veins (Figures 6A,B). The gene discussed is VEGFA; the disease is brain ischemia.