According to the subgroup analysis, a statistically significant association was identified in the tumor budding subgroup for E‐cadherin expression positivity (HR: 0.026, 95% CI: 0.002–0.324, p = 0.005), tumor stage (HR: 3.306, 95% CI: 1.037–10.538, p = 0.043), and differentiation grade (HR: 0.029, 95% CI: 0.002–0.432, p = 0.010). This evidence concerns the gene CDH1 and neoplasm.