Photodynamic therapy (PDT) is a promising cancer treatment.Thepresent work used two toluidine blue O (TBO) derivatives covalentlyconjugated to human serum albumin (HSA) as a drug delivery systemand bound to cucurbit[7]­uril (CB[7]) as a protective nanocapsule inorder to study their effect of cellular uptake and phototoxicity inHeLa cells. The gene discussed is ALB; the disease is cancer.