CAD and neoplasm: To further examine the relevance of this finding in vivo, we stained Cad-KO and control Panc02 tumor cells (Td-Tomato+) with Annexin V. Consistent with the in vitro findings, Cad KO tumor cells exhibited higher levels of Annexin V+ cells (gating based on PI-negative cells) compared with control tumor cells (Figure 4, H and I, and Supplemental Figure 4J).