Multivariate COX regression analysis showed that the positive expression of KAI1, MACC1, and AGR2, TNM stage and lymph node metastasis stage in clinicopathological factors were independent prognostic factors for the OS time of patients with cervical squamous cell carcinoma (P < .05). This evidence concerns the gene MACC1 and metastatic malignant neoplasm in the lymph nodes.