Lymph node metastasis (LNM) served as an indicator of the tumor’s malignant biological behavior, with positive AGR2 expression noted in 18 out of 68 patients (18/68, 26.5%) classified as N0 and in 21 out of 38 patients (21/38, 55.3%) classified as N1, demonstrating a significant difference in AGR2 expression relative to lymph node metastasis (χ2 = 9.138, P < .001). This evidence concerns the gene AGR2 and metastatic malignant neoplasm in the lymph nodes.