Furthermore, the double-positive and non-double-positive groups revealed significant differences in tumor diameter, AFP, GGT, and differentiation grade (P < 0.05), with no significant differences observed in gender, age, hepatitis status, AST, ALT, NC, LYC, PLC, NLR, or PLR (P > 0.05) (Tables 1 and 2). This evidence concerns the gene AFP and Hepatitis.