Therefore, this study was designed to identify and classify the Ganoderma ellipsoideum VNES22015-A1 collected in Vietnam using both morphological characteristics and molecular phylogenetic analysis based on ITS rDNA sequencing, and evaluate the potential anticancer activity of its ethanol extract and solvent-partitioned fractions against human breast cancer MCF-7 cells using in vitro cytotoxic assays and in silico molecular docking against key breast cancer-related targets (HPA, MELK, CK2α, and NUDT5). Here, MELK is linked to breast carcinoma.