Upon subcutaneous tumor establishment with volumes ranging from 50–100 mm3, animals were systematically randomized into three experimental cohorts (n=5 per group): a control cohort administered phosphate-buffered saline (PBS), a positive control group receiving anti-PD-L1 monoclonal antibody (5 mg kg-1), and experimental group receiving SA treatment (5 mg kg-1). This evidence concerns the gene CD274 and neoplasm.