Based on the discovery of Sal and CK1α ′s role in the regulation of the autophagic pathway in RAS-driven cancer, and in addition to the fact that autophagy and ferroptosis are two essential survival stress-management pathways for colorectal cancer, we evaluated the effect of Sal and CK1α inhibitor on autophagy and ferroptosis in HCT116 CRC cells in vitro by measuring cell survival, cell migration, and the expression levels of MMP-2 and MMP-9, and autophagy-associated genes, Beclin1, LC3βII, and p62, along with the intracellular MDA concentration and GSH/GSSG ratio measurement. Here, SQSTM1 is linked to cancer.