In addition, this combination resulted in a synergistic depletion of GSH and production of MDA, as well as an inhibition of autophagic flux by upregulating the gene expressions of Beclin1, LC3βII, and p62. In conclusion, the combination of Sal and D4476 suppressed the growth of HCT116 CRC cells by inducing ferroptosis and inhibiting autophagic flux. The gene discussed is SQSTM1; the disease is colorectal carcinoma.