While conventional asparaginases often lack direct cytotoxic effects on tumor cells [80, 81], our study demonstrates that purified L-asparaginase from S. acroporae S4-41 exhibits significant dose-dependent cytotoxicity against multiple cancer cell lines, with IC50 values of 513 μg/mL (NB4), 737.4 μg/mL (HepG-2), and 711.5 μg/mL (MCF-7). This evidence concerns the gene ASPG and neoplasm.