In utero electroporation (IUE) of this cargo plasmid along with a GLAST-driven hyperactive piggyBac transposase (hyPBase) (Yusa et al., 2011), together with a self-excising iCreI plasmid (pCAGEN-se-iCreI-HA), resulted in stable transgene expression in radial glia descendants and frank glioma, as evidenced by extensive upregulation of reactive astrocytes shown by immunohistochemistry for anti-glial fibrillary acidic protein (GFAP) (Fig. 8B-D). The gene discussed is SLC1A3; the disease is glioma.