To further confirm that astrapterocarpan‐mediated TRAF6 inhibition constitutes a key mechanism underlying JBD's protective effects against LPS‐induced ALI, we analysed the expression levels of key TLR4 pathway components (TRAF6, TLR4, MyD88 and IRAK4) in alveolar macrophages isolated from the murine model. This evidence concerns the gene TLR4 and acute respiratory distress syndrome.