TNF and Thrombocytopenia: Previous studies have consistently reported that NETosis is activated in sepsis, often in association with inflammatory biomarkers (e.g. plasma CRP, PCT, IL-6 and TNFα), dysregulated coagulation responses (e.g. elevated D-dimer, prolonged prothrombin time and thrombocytopenia) and indicators of tissue damage (e.g. increased LDH) (23–27, 33).