The alcian blue staining on RKO tumor Formalin-Fixed Paraffin-Embedded (FFPE) section slides, with or without the pre-treatment of HYAL-Fc or EGFR × HYAL before the staining, demonstrated that both HYAL-Fc and EGFR × HYAL effectively degraded HA in the RKO tumor stromata (Figure 2A). Here, EGFR is linked to neoplasm.