Furthermore, LRA supplementation has been demonstrated to improve hepatic gluconeogenesis and IR in mice with high-fat diet-induced obesity, which was achieved by decreasing the protein expression of toll-like receptor 4 (TLR4)/NF-κB/JNK signal pathway to ameliorate inflammation and activating the Nrf2/HO-1/(NQO1) NAD(P)H: quinone oxidoreductase 1 pathway to reduce oxidative stress in hepatic tissues [83]. The gene discussed is TLR4; the disease is obesity due to melanocortin 4 receptor deficiency.