Their analysis focused on the expression of key genes associated with the Rho/ROCK signaling pathway, cytoskeleton, and apoptosis, including ITGB1 (Integrin β-1), Rho (Rhodopsin), ROCK (Rho-associated coiled-coil containing protein kinase 1), MLC-1 (Megalencephalic leukoencephalopathy with subcortical cysts 1), VIM-1 (Vimentin), Bax, and Bcl-2. Here, RHO is linked to megalencephalic leukoencephalopathy with subcortical cysts.