Therefore, the aim of our present study was to examine (I) whether AGEs mediate an epigenetic alteration on the repressive histone 3 mark H3K27me3 in proximal tubular cells and (II) are EZH2 expression and H3K27me3 levels affected from sex-difference in renal tubules of non-diabetic and db/db mice, a well-established type 2 diabetes model [24]. Here, EZH2 is linked to type 2 diabetes mellitus.