In this study, four representative cell line models relevant to AML research (i.e., HL-60, HEL, THP-1 and K562) were selected to determine the relevance of DGKα and DGKζ in AML cell viability using both isoform-specific chemical inhibitors and siRNA-mediated gene silencing. The gene discussed is DGKZ; the disease is acute myeloid leukemia.